In the following example, the Vibro® Technology is applied in an extracellular API/protein process where the fermentation broth is processed directly, and a concentrated API/protein is achieved.
Description:
The fermentation broth is processed in a Vibro-I microfiltration unit where the cells are separated from the API/protein. The resulting cell concentrate is returned to the harvest tank. The positive pump creates an ultralow TMP that prevents fouling from building up and as a consequence a high transmission of API/protein is maintained throughout the process. The cell-free API/protein from the Vibro-I microfiltration unit is transferred to the product tank.
From the product tank, the API/protein is gently concentrated in a Vibro-I ultrafiltration unit where the concentrate is returned to the product tank. A positive pump creates the pressure needed for the process and can return the API free buffer to the harvest tank to wash the cells further, while keeping the cell concentration low.
When the fermentation broth is virtually API/protein free, the Vibro-I microfiltration unit is stopped and the Vibro-I ultrafiltration unit is run until the API/protein concentration target is reached.
It is also possible to change the buffer, adjust the pH etc. during the process.
The setup makes it possible to achieve up to 95% recovery without using additional liquid for diafiltration.
The system is simple to use and requires minimal control.