In the following example, the Vibro® Membrane Filtration (VMF) technology is used for the critical solid/liquid separation following cultivation of mammalian cells.
Description:
Vibro® technology has proven to be an effective means of cell removal from cultivation broth, both in microbial and mammalian processes.
The continuous cleaning effect of the vibrating membrane allows for high flux and the wide offering of membrane materials offer the possibility of optimization of product transmission tailored to the individual cultivation process. Compared to dead-end filtration, Vibro® filtration also increases efficiency by enabling diafiltration.
Mammalian cells, such as CHO, which are considered to be especially fragile, can be retained without indications of cell damage, decrease in viability or release of Host Cell Proteins during filtration procedures of up to 8 hours.
Other applications of the Vibro® Membrane Filtration (VMF) technology besides harvest in bioreactors include:
- Ultrafiltration of harvest material to reduce volume before further processing.
- Cell retention device in perfusion processes
- Recovery of product from perfusion bleed streams