Single-Pass VMF mAbs

Single-Pass Vibro^® Membrane Filtration (VMF) of mAbs in a single step

The Single-Pass VMF outperforms conventional SPTFF with minimal fouling, high concentration efficiency, and gentle, stable operation. Its innovative anti-fouling mechanism enables true Single-Pass concentration in a single step — a breakthrough in continuous bioprocessing.

Reduced DSP cost | Single-Pass concentration

DSP remains a major cost driver in mAb production, accounting for up to 90% of total costs, with Protein A chromatography among the most expensive steps.

By pre-concentrating the dilute harvest pool before Protein A capture, productivity and loading capacity increase while buffer consumption drops significantly.

Single-Pass concentration offers a smart, streamlined DSP strategy that cuts costs and simplifies operation.

Current challenges diminished

Even optimized conventional TFF setups often require multiple devices in series to reach target concentration. This study demonstrates the suitability of Vibro^® VMF for true Single-Pass, Single-Step processing — reaching target concentration over a single device. 

Enabling Single-Pass Single-Step with Vibro^® VMF

Using Human Serum Immunoglobulin G (hIgG) as a model, Vibro^®VMF achieved:

• up to 20X concentration from a 10 g/L feed
• up to 10X from a 20 g/L feed
• flux at 17.2 LMH under stable and uniform TMP

These results highlight the superior performance and scalability of Vibro^®VMF in a Single-Pass, Single-Device operation.

Stable, long term process technology | DSP intensification

Extended continuous runs confirmed the VMF robustness:

• 10X concentration for over 6 hours run ( 10 g/L feed)
• 5X concentration for more than 8 hours run ( 20 g/L feed)
• no aggregation or fragmentation (verified by dynamic light scattering)

Vibro^® VMF delivers gentle high-efficiency Single-Pass concentration, eliminating the need for multi-stage configurations and paving the way for DSP intensification.