VMF of E. coli Cultures & Lysates
VMF performs better than conventional TFF for the processing of high‑density E. coli cultures and lysates, making it a robust, high-performance solution for demanding midstream applications.
Helena Maier
Mass transfer characteristics of VMF Protein UF
VMF technology delivers a step change in protein ultrafiltration performance. It enables higher productivity, lower operating pressure and improved process robustness in bioprocessing, particularly for the concentration of monoclonal antibodies (mAbs) and other high‑value protein products.
Single-Pass VMF of mAbs
Single-pass VMF outperforms conventional single-pass TFF in the concentration of mAbs, with minimal fouling, high concentration and conversion efficiency and gentle, stable operation.
API/Protein Concentration & Recovery
In the following example, the Vibro® Technology is applied in an extracellular API/protein process where the fermentation broth is processed directly, and a concentrated API/protein is achieved. Description: The fermentation broth is processed in a Vibro-I microfiltration unit where the cells are separated…
Single-Step Cell Concentration & API Recovery
In this example, a fermentation broth containing cells such as E. coli bacteria is being harvested and concentrated directly from the harvest tank using VMF technology. At the same time, the fermentation broth is processed and a cleaned API/protein is…
Concentration of Skim Milk to Greek Yoghurt
In the following example, the Vibro® Technology is applied to a Greek Yoghurt process where the cultured skim milk is concentrated to Greek Yoghurt. Description: The cultured milk is processed continuously using a multi-step setup. In all three steps, the retentate…
Algae Harvest & Concentration
Vibro® Membrane Filtration (VMF) enables the continuous and highly efficient harvest and concentration of algae. Within just a three steps, concentrations of 200 g/l from a constant stream of 6500 l/h can be reached. A similar process can be used to…
Beer Brewing
In this example, VMF is applied in brewery operations, where it has the potential to be used in a range of applications and can replace a variety of conventional technologies such as High Speed Separators (HSS), with both qualitative and…
Perfusion process Intensification
In this example, the Vibro® Membrane Filtration (VMF) technology is used as perfusion device to retain cells in the bioreactor and to harvest API in the permeate from VMF1. Description: In upstream processing, Vibro Membrane Filtration (VMF) can act as…
Harvest of Mammalian Cell Cultivations
In the following example, the Vibro® Membrane Filtration (VMF) technology is used for the critical solid/liquid separation following cultivation of mammalian cells. Description: Vibro® technology has proven to be an effective means of cell removal from cultivation broth, both in microbial and…